scaav sgrna gfp  (Addgene inc)

Journal: Bioactive Materials

Article Title: A near-infrared regulated programmable multi-mode periosteum scaffold for sequential healing of infected bone defects

doi: 10.1016/j.bioactmat.2026.03.046

Figure Lengend Snippet: Antimicrobial activity of multi-mode periosteum scaffolds with different NIR irradiation strategies. A. Representative images of MRSA and E. coli colonies with different treatments. Quantitative analysis of MRSA ( B ) and E. coli ( C ) colonies in (A), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. Ns ( p > 0.05), ∗∗ p < 0.01. D. Representative SEM images of MRSA and E. coli colonies with different treatments. Scale bar: 1 μm. E. Live/dead staining of MRSA and E. coli colonies in different treatment groups. Scale bar: 100 μm. F. Confocal microscopy image of MRSA biofilm. Scale bar: 100 μm. Protein leakage statistics for MRSA ( G ) and E. coli ( H ), respectively (n = 3). Results are presented as means ± SD. Samples were subjected to one-way ANOVA with Tukey's post hoc test. ∗∗ Significant differences of group M + CI vs. NC, M, and M + II, p < 0.01.

Article Snippet: Methicillin-resistant Staphylococcus aureus (MRSA, ATCC 43300) and Escherichia coli (ATCC 25922) were purchased from the American Type Culture Collection (ATCC).

Techniques: Activity Assay, Irradiation, Staining, Confocal Microscopy

Journal: The Journal of Physiological Sciences : JPS

Article Title: Dynamic regulation of TASK-1 channels under cellular stress

doi: 10.1016/j.jphyss.2026.100069

Figure Lengend Snippet: Effect of chemical hypoxia on cell surface TASK-1 localization. (A) The top panel shows nuclear staining with Hoechst 33342 (blue) and membrane labeling with WGA (red) in cells that express TASK-1-GFP. The lower panels present representative fluorescence images of TASK-1 from cells that were transfected with TASK-1-GFP treated with NaCN and/or BIM. Scale bar indicates 10 µm. (B) Summary of the intensity of TASK-1 fluorescence which was measured in the periphery of cells that express TASK-1-GFP. Cells were treated with NaCN, BIM, or both. Results are normalized to the control group. Experiments were conducted 3 times. (C) Diagram illustrating TASK-1 membrane topology with an extracellular HA tag used for chemiluminescence measurements. (D) Measurement of plasma membrane chemiluminescence of TASK-1 in cells treated with NaCN, BIM, or a combination. Data were normalized to control values and obtained from 3 separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The 263–264 LL-AA mutant of TASK-1-GFP was synthesized by Genewiz (South Plainfield, NJ).

Techniques: Staining, Membrane, Labeling, Fluorescence, Transfection, Control, Clinical Proteomics

Journal: The Journal of Physiological Sciences : JPS

Article Title: Dynamic regulation of TASK-1 channels under cellular stress

doi: 10.1016/j.jphyss.2026.100069

Figure Lengend Snippet: Involvement of dynamin in PKC-dependent TASK-1 endocytosis. (A) Images showing colocalization of TASK-1 and WGA in cells with or without NaCN and BIM treatment. Scale bar indicates 10 µm. (B) Representative TASK-1 images from cells expressing TASK-1-GFP with or without co-expressing dynamin K44E in the absence or presence of NaCN. Scale bar = 10 µm. (C) Measurement of TASK-1 fluorescence at the surface membrane of cells expressing TASK-1-GFP co-expressing or lacking dynamin K44E with or without NaCN treatment. Data are relative to the control value. Data were gathered from 4 separate experiments. **p < 0.01, ***p < 0.001.

Article Snippet: The 263–264 LL-AA mutant of TASK-1-GFP was synthesized by Genewiz (South Plainfield, NJ).

Techniques: Expressing, Fluorescence, Membrane, Control

Journal: The Journal of Physiological Sciences : JPS

Article Title: Dynamic regulation of TASK-1 channels under cellular stress

doi: 10.1016/j.jphyss.2026.100069

Figure Lengend Snippet: Impact of cholesterol depletion on cell surface TASK-1 expression . (A) Representative TASK-1 images from cells expressing TASK-1-GFP. Cells were treated with NaCN following MβCD pretreatment. Scale bar indicates 10 µm. (B) Quantitative summary of TASK-1 fluorescence at the cell membrane following treatments with MβCD, NaCN, MβCD + NaCN, or cholesterol-saturated MβCD + NaCN. Data were normalized to control values and obtained from 3 separate experiments. *p < 0.05.

Article Snippet: The 263–264 LL-AA mutant of TASK-1-GFP was synthesized by Genewiz (South Plainfield, NJ).

Techniques: Expressing, Fluorescence, Membrane, Control

Journal: The Journal of Physiological Sciences : JPS

Article Title: Dynamic regulation of TASK-1 channels under cellular stress

doi: 10.1016/j.jphyss.2026.100069

Figure Lengend Snippet: Role of a double-leucine based motif in TASK-1 endocytosis. (A) TASK-1 membrane topology with double-leucine residues (underlined) mutated. (B) Fluorescence images of TASK-1 in cells transfected with wild type TASK-1-GFP or mutant TASK-1-GFP (263–264LL-AA) under control or in the presence of NaCN. Scale bar: 5 µm. (C) Quantitative summary of TASK-1-GFP fluorescence at the cell membrane of wild type or double-leucine mutant TASK-1-GFP. Cells were treated with or without NaCN. Data are normalized to the control (left and middle) or wild type group (right). The experiments were repeated three times. **p < 0.01.

Article Snippet: The 263–264 LL-AA mutant of TASK-1-GFP was synthesized by Genewiz (South Plainfield, NJ).

Techniques: Membrane, Fluorescence, Transfection, Mutagenesis, Control

Journal: The Journal of Physiological Sciences : JPS

Article Title: Dynamic regulation of TASK-1 channels under cellular stress

doi: 10.1016/j.jphyss.2026.100069

Figure Lengend Snippet: Effect of PKC activation on cell surface TASK-1 localization and endocytosis (A) Representative fluorescence images of TASK-1 from cells that were transfected with TASK-1-GFP treated with PMA and/or BIM. Scale bar indicates 10 µm. (B) Summary of the intensity of TASK-1 fluorescence which was measured in the peripheral regions of cells that express TASK-1-GFP. Cells were treated with PMA, BIM, or both. (C) Measurement of plasma membrane chemiluminescence of TASK-1 in cells treated with PMA, BIM, or a combination. (D) Measurement of TASK-1 fluorescence at the surface membrane of cells expressing TASK-1-GFP co-expressing or lacking dynamin K44E with or without PMA treatment. All quantitative data were normalized to control value. Data from each type of experiments (B, C, and D) were collected from three separate experiments. *p < 0.05, **p < 0.01, ***p < 0.001.

Article Snippet: The 263–264 LL-AA mutant of TASK-1-GFP was synthesized by Genewiz (South Plainfield, NJ).

Techniques: Activation Assay, Fluorescence, Transfection, Clinical Proteomics, Membrane, Expressing, Control